abstract：:Colorectal cancer is the second leading cause of malignant death, and better preventive strategies are needed. The treatment of colonic cancer remains difficult because of the lack of effective chemotherapeutic agents; therefore it is important to continue to search for cellular functions that can be disrupted by chem...

journal_title：International journal of molecular medicine

authors： Selvakumar P,Pasha MK,Ashakumary L,Dimmock JR,Sharma RK

更新日期：2002-10-01 00:00:00

abstract：:Elastofibroma is a rare, benign, slow-growing degenerative pseudotumor that typically occurs in the subscapular region and has been considered a peculiar fibroblastic proliferation with accumulation of abnormal elastic fibers. Very little is known about the cytogenetic and molecular genetic changes in elastofibroma. I...

journal_title：International journal of molecular medicine

authors： Nishio JN,Iwasaki H,Ohjimi Y,Ishiguro M,Koga T,Isayama T,Naito M,Kikuchi M

更新日期：2002-09-01 00:00:00

abstract：:Heparanase is an enzyme that degrades extracellular glycoprotein to release heparan sulfate molecules. CD44 variant exon 3 (CD44v3), a receptor for heparan sulfate, generates intracellular signals for cell migration. Production of CD44v3 and expression of heparanase were detected by immunohistochemistry and in situ hy...

journal_title：International journal of molecular medicine

authors： Kuniyasu H,Chihara Y,Kubozoe T,Takahashi T

更新日期：2002-09-01 00:00:00

abstract：:Human skin fibroblasts may be the target cells for estrogens. The aim of present study was to confirm the presence of both isoforms of estrogen receptors (ER) in these cells. Experiments were carried out in primary cultures of human skin fibroblasts. ER-alpha and ER-beta mRNAs were measured by quantitative assays base...

journal_title：International journal of molecular medicine

authors： Haczynski J,Tarkowski R,Jarzabek K,Slomczynska M,Wolczynski S,Magoffin DA,Jakowicki JA,Jakimiuk AJ

更新日期：2002-08-01 00:00:00

abstract：:Telomerase activity and hTERT mRNA expression are upregulated in colorectal cancer. Whether they are inherent in colorectal adenomas, premalignant lesions to cancer, however, remains to be elucidated. We examined telomerase activity by the fluorescence-based telomeric repeat amplification protocol method and analyzed ...

journal_title：International journal of molecular medicine

authors： Kanamaru T,Tanaka K,Kotani J,Ueno K,Yamamoto M,Idei Y,Hisatomi H,Takeyama Y

更新日期：2002-08-01 00:00:00

abstract：:Many viruses have been investigated for their oncolytic properties and potential use as therapeutic agents for cancer treatment. Most of these replication-competent viruses are human pathogens. We investigated the oncolytic properties of an animal virus which is non pathogenic for both its natural host and humans. Bov...

journal_title：International journal of molecular medicine

authors： Smyth M,Symonds A,Brazinova S,Martin J

更新日期：2002-07-01 00:00:00

abstract：:Gene therapy is becoming an important treatment modality against various diseases including cancer, genetic disorders, infectious diseases and inflammatory diseases. For achieving successful gene therapy, it is extremely important to control the expression of a transduced therapeutic gene. However, there have been few...

journal_title：International journal of molecular medicine

authors： Akahane M,Kuriyama S,Ohgushi H,Akahane T,Kawamura K,Watanabe S,Funakoshi F,Yoshiji H,Ikenaka K,Takakura Y

更新日期：2002-07-01 00:00:00

abstract：:The pathogenesis of inflammatory bowel disease (IBD) remains unknown. It has been suggested that luminal factors such as the microflora, short chain fatty acids (SCFAs), food antigens and so on play important roles in the disease progression. Many reports have revealed alterations in the SCFA and organic acid concentr...

journal_title：International journal of molecular medicine

authors： Araki Y,Andoh A,Fujiyama Y,Itoh A,Bamba T

更新日期：2002-06-01 00:00:00

abstract：:We examined whether generation of H2O2 is a critical event for the apoptotic pathway upstream of mitochondrial involvement and caspase-3 protease activation. Perylquinone photosensitizers such as Hypocrellin A (HA), Hypocrellin B (HB) and Hypericin (HY) induced activation of caspase-3 and apoptosis upon photoactivatio...

journal_title：International journal of molecular medicine

authors： Ali SM,Chee SK,Yuen GY,Olivo M

更新日期：2002-05-01 00:00:00

abstract：:Histone deacetylase inhibitor (HDAI) induces accumulation of highly acetylated histones by inhibiting the activity of histone deacetylase and inhibits cell proliferation, induces differentiation, and promotes apoptosis. TNF-related apoptosis inducing ligand (TRAIL) induces apoptosis in various human cancer cells, a pr...

journal_title：International journal of molecular medicine

authors： Inoue H,Shiraki K,Ohmori S,Sakai T,Deguchi M,Yamanaka T,Okano H,Nakano T

更新日期：2002-05-01 00:00:00

abstract：:p57 (Kip2) belongs to the Cip/Kip family and is one of the universal negative regulators of the cell cycle. In this study, we investigated the p57 expression of various types of thyroid neoplasm. p57 overexpression was observed in only 4.2% of normal thyroid tissues. In follicular adenoma and minimally invasive follic...

journal_title：International journal of molecular medicine

authors： Ito Y,Yoshida H,Nakano K,Kobayashi K,Yokozawa T,Hirai K,Matsuzuka F,Matsuura N,Kuma K,Miyauchi A

更新日期：2002-04-01 00:00:00

abstract：:We have shown previously that soluble low-molecular-mass tumor-associated antigens (sTAA) promote the anti-tumor effect of the anticancer drug cyclophosphamide (CPA) on rat mammary carcinogenesis. In this study, we analyzed the possible mechanism underlying this phenomenon. Studies were performed on tumors obtained fr...

journal_title：International journal of molecular medicine

authors： Ben-Hur H,Kossoy G,Zandbank J,Zusman I

更新日期：2002-04-01 00:00:00

abstract：:Serum concentration levels of des-gamma-carboxy prothrombin (DCP), alpha-fetoprotein (AFP) and Lens culinaris agglutinin-reactive fraction (AFP-L3) are useful tumor markers for the diagnosis of hepatocellular carcinoma (HCC). Recently, a novel immunoassay using the electrochemiluminescence (ECLIA) was developed to ena...

journal_title：International journal of molecular medicine

authors： Shimizu A,Shiraki K,Ito T,Sugimoto K,Sakai T,Ohmori S,Murata K,Takase K,Tameda Y,Nakano T

更新日期：2002-03-01 00:00:00

abstract：:Adrenomedullin (ADM) is a hypotensive peptide, that derives from the proteolytic cleavage of pro(p)ADM and acts through two subtypes of receptors, called L1-receptor (L1-R) and calcitonin receptor-like receptor (CRLR). CRLR may function as a calcitonin gene-related peptide or a selective ADM receptor depending on the ...

journal_title：International journal of molecular medicine

authors： Rebuffat P,Forneris ML,Aragona F,Ziolkowska A,Malendowicz LK,Nussdorfer GG

更新日期：2002-02-01 00:00:00

abstract：:The aetiological agent of chronic hepatitis C is the hepatitis C virus. The hepatitis C virus is spread by parenteral transmission of body fluids, primarily blood or blood products. In 1989, after more than a decade of research, HCV was isolated and characterised. The hepatitis C viral genome is a positive-sense, sing...

journal_title：International journal of molecular medicine

authors： Fanning LJ

更新日期：2002-02-01 00:00:00

abstract：:A large number of p53-transcribed proteins have been shown to mediate growth arrest and/or apoptosis in vitro, whereas their in vivo roles remain largely unclear. p53 is capable of initiating apoptosis without transcription of apoptosis inducer genes, although the underlying mechanism is unknown. p53 is present in the...

journal_title：International journal of molecular medicine

authors： Chang NS

更新日期：2002-01-01 00:00:00

abstract：:Antigen presenting cells, especially the antigen presenting dendritic cells (DC) in the tissue, regulate the magnitude of antigen-specific immune response. A role of impaired and narrowly focused specific immune response has been implicated in the pathogenesis of chronic hepatitis due to hepatitis B virus and hepatiti...

journal_title：International journal of molecular medicine

authors： Abe M,Kajino K,Akbar SM,Yamamura K,Onji M,Hino O

更新日期：2002-01-01 00:00:00

abstract：:The elevation of natural killer cell activity (NKCA) by laughter was not confirmed due to incomplete methodology of previous studies although positive emotion is believed to be favorable for health. To verify NKCA elevation by laughter in a crossover design, we measured NKCA before and after watching films, presenting...

journal_title：International journal of molecular medicine

authors： Takahashi K,Iwase M,Yamashita K,Tatsumoto Y,Ue H,Kuratsune H,Shimizu A,Takeda M

更新日期：2001-12-01 00:00:00

abstract：:The molecular cloning and sequencing of the cDNA coding for a novel regucalcin gene promoter region-related protein (RGPR) was investigated using rat, mouse and human liver cDNA library with a yeast one-hybrid system and a rapid amplification of cDNA ends (RACE) method. The clone coding an unknown protein was isolated...

journal_title：International journal of molecular medicine

authors： Misawa H,Yamaguchi M

更新日期：2001-11-01 00:00:00

abstract：:Formalin-fixed, paraffin-embedded (FFPE) tissues are one of the popular sources of diagnostic materials, the easiest to store and transport. They are often used as the source of nucleic acids for retrospective molecular analyses based on DNA amplification by polymerase chain reaction (PCR). However, it is known that n...

journal_title：International journal of molecular medicine

authors： Bielawski K,Zaczek A,Lisowska U,Dybikowska A,Kowalska A,Falkiewicz B

更新日期：2001-11-01 00:00:00

abstract：:Immortalized cloned human chondrocytes isolated from a normal (Ch-4, 8, N) and an osteoarthritis patient (Ch-8-OA) were established by introduction of recombinant SV40-adenovirus vector containing SV40 early gene. These cells exhibited continuous proliferative capacity in monolayer culture and showed chondrocytic char...

journal_title：International journal of molecular medicine

authors： Yoshimatsu T,Saitoh A,Ryu JN,Shima D,Handa H,Hiramoto M,Kawakami Y,Aizawa S

更新日期：2001-10-01 00:00:00

abstract：:Calcium channel blocker is useful for a variety of purposes and is effective for preventing hepatitis elicited by different inducers, suggesting its possible clinical application for treating hepatitis. The alpha1-subunit of the dihydropyridine-sensitive L-type calcium channel is a target of calcium channel blocker. F...

journal_title：International journal of molecular medicine

authors： Okamoto T,Jeong SY,Takahashi Y,Baughman KW,Ogata K,Goto J,Kanazawa I

更新日期：2001-10-01 00:00:00

abstract：:p38 has been shown to be involved in TGF-beta-induced gene expression, but the upstream of the signaling pathway leading to the activation of p38 is left undefined. We investigated the pathway in cultured human keratinocytes (HaCat cells). Western blot analysis revealed that TGF-beta induced the activation of p38 with...

journal_title：International journal of molecular medicine

authors： Chiu C,Maddock DA,Zhang Q,Souza KP,Townsend AR,Wan Y

更新日期：2001-09-01 00:00:00

abstract：:The activation of the protease-activated receptor 1 (PAR-1) by thrombin has been shown to induce an activation of the MAP kinase cascade and to stimulate cell proliferation. To examine the mechanisms of signal transduction by PAR-1, we constructed several PAR-1 mutants which were stably expressed in CHO cells. When co...

journal_title：International journal of molecular medicine

authors： Chen X,Berrou J,Vigneau C,Rondeau E

更新日期：2001-09-01 00:00:00

abstract：:The uptake of D-[3H]mannoheptulose by isolated pancreatic islets was recently proposed as a tool to assess the relative contribution of insulin-producing cells to the total mass of the islets. In the present study, the uptake of the tritiated heptose over 60 min incubation at 37 degrees C was about 21% lower in islets...

journal_title：International journal of molecular medicine

authors： Sener A,Ladriere L,Malaisse WJ

更新日期：2001-08-01 00:00:00

abstract：:This study aimed to evaluate the in vitro effect of lactoperoxidase with or without its substrates (hydrogen peroxide, thiocyanate) on the growth of 4 different black pigmented anaerobe (BPA) strains associated with the development and progress of periodontal diseases: Porphyromonas gingivalis ATCC 33277, Prevotella i...

journal_title：International journal of molecular medicine

authors： Fadel M,Courtois P

更新日期：2001-07-01 00:00:00

abstract：:The tumor suppressor genes p15INK4B and p16INK4A, located in the chromosomal region 9p21, are frequently inactivated by homo- or hemizygous deletions, point mutation or promotor methylation in various types of cancer. No commercial probe is yet available that allows the detection of such deletions by FISH. Long distan...

journal_title：International journal of molecular medicine

authors： Bleichert A,Fiedler W,Claussen U,Ernst G,Loncarevic IF,Heller A,Liehr T,Kunert C,von Eggeling F

更新日期：2001-06-01 00:00:00

abstract：:To analyse the mechanisms of PAR-1 internalisation, we constructed several PAR-1 mutants and stably expressed them in CHO cells. Our study shows that the Ser(306)-->Ala mutation (S306A), which eliminates a potential site of phosphorylation by PKC in the third intracellular loop of PAR-1, did not change the rate of pho...

journal_title：International journal of molecular medicine

authors： Chen X,Berrou J,Vigneau C,Delarue F,Rondeau E

更新日期：2001-06-01 00:00:00

abstract：:D-mannoheptulose was recently proposed as a tool to label preferentially insulin-producing cells in the pancreatic gland in the perspective of the non-invasive imaging of the endocrine pancreas. In such a perspective, we have now synthesized 1-deoxy-1-[125I]iodo-D-mannoheptulose ([125I]MH) and examined its uptake by d...

journal_title：International journal of molecular medicine

authors： Sener A,Bessieres B,Courtois P,Ladriere L,Louchami K,Jijakli H,Malaisse WJ

更新日期：2001-05-01 00:00:00

abstract：:We reviewed the effectiveness of commercial serological markers for the early detection of cancer and monitoring cancer patients. Parameters, such as specificity and variability of tumor markers were compared with a new approach for cancer detection which was recently developed in our laboratory. We demonstrate that t...

journal_title：International journal of molecular medicine

authors： Zusman I,Ben-Hur H

更新日期：2001-05-01 00:00:00

abstract：:Transporters such as P-glycoprotein (MDR1), multidrug resistance protein 1 (MRP1), lung resistance-related protein (LRP) and breast cancer resistance protein (BCRP) are associated with multidrug resistance in various carcinoma cell lines. The expression of these molecules has been also characterized in human normal ti...

journal_title：International journal of molecular medicine

authors： Takebayashi Y,Nakayama K,Fujioka T,Kanzaki A,Mutho M,Uchida T,Miyazaki K,Ito M,Fukumoto M

更新日期：2001-04-01 00:00:00

abstract：:Bcl-2 family member proteins are differentially expressed in skin and in non-melanoma skin cancer (NMSC). To elucidate the contribution of bcl-2 and bax proteins to epidermal differentiation and skin carcinogenesis, we investigated keratinocyte proliferation, differentiation and tumourigenesis in bcl-2(-/-) and bax(-/...

journal_title：International journal of molecular medicine

authors： Cho SH,Delehedde M,Rodriguez-Villanueva J,Brisbay S,McDonnell TJ

更新日期：2001-03-01 00:00:00

abstract：:Free radicals derived from the reaction of iron and oxygen are thought to be one of the causes of tissue injury. In order to identify whether oxygen concentrations are an important factor in iron-mediated damage to cells, cytotoxic effects of Fe(3+)-NTA on human fibroblasts (KMST-6 line) were studied under the conditi...

journal_title：International journal of molecular medicine

authors： Pu H,Sakaguchi M,Kondo T,Kondo A,Kawabata T,Namba M

更新日期：2001-03-01 00:00:00

abstract：:Anorexia that develops in chronic hepatitis is associated with cytokine expression in the brain. Treatment of mice with concanavalin A (12.5 mg/kg, i.v.) elevated the plasma alanine aminotransferase activity at 8.5 h after treatment. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta mRNA expression was ind...

journal_title：International journal of molecular medicine

authors： Okamoto T,Okabe S

更新日期：2001-02-01 00:00:00

abstract：:Whether or not nutritionally-regulated foreign gene expression in vivo is achievable was examined in mouse liver after in vivo gene transfer by electroporation (EP). Electric pulses were applied to a left liver lobe immediately after injection of a luciferase reporter gene driven by the liver-type phosphoenolpyruvate ...

journal_title：International journal of molecular medicine

authors： Muramatsu T,Ito N,Tamaoki N,Oda H,Park HM

更新日期：2001-01-01 00:00:00

abstract：:Type I interferon (IFN) receptor consists of two chains (Hu-IFN-alphaR1 and Hu-IFN-alphaR2), and Hu-IFN-alphaR2 takes a soluble, short, or long form (Hu-IFN-alphaR2a, Hu-IFN-alphaR2b, or Hu-IFN-alphaR2c, respectively). We examined Hu-IFN-alphaR2 expression in hepatocellular carcinoma (HCC) tissues and their correspond...

journal_title：International journal of molecular medicine

authors： Takayama A,Yano H,Ogasawara S,Higaki K,Kojiro M

更新日期：2000-12-01 00:00:00

abstract：:The colonic crypt contains highly proliferative cells in its base and differentiated cells on its luminal surface. Carcinogenesis significantly affects this orderly cellular distribution. The aims of this study were: i) to examine the expression of apoptosis-related proteins along the crypt-lumen axis during 1, 2-dime...

journal_title：International journal of molecular medicine

authors： Avivi-Green C,Madar Z,Schwartz B

更新日期：2000-12-01 00:00:00

abstract：:S100A4, one of the tandemly arranged S100 genes at chromosome 1q21, has been suggested to play a functional role in cell motility and invasiveness of tumor cell growth. We investigated the expression of S100A4 and the in vitro invasiveness of 4 human bile duct adenocarcinoma cell lines by the Matrigel assay. S100A4 wa...

journal_title：International journal of molecular medicine

authors： Katayama N,Murao S,Ajiki T,Kitazawa S,Onoyama H,Kuroda Y,Maeda S

更新日期：2000-11-01 00:00:00

abstract：:In hypercholesterolemia increased lipid and lipoprotein peroxidation occurs. The renin-angiotensin system plays an important role in atherogenesis. Angiotensin II induces smooth muscle cells proliferation and stimulates oxidation of LDL particles and foam cell accumulation. Inhibition of ang II production leads to dec...

journal_title：International journal of molecular medicine

authors： Wojakowski W,Gminski J,Siemianowicz K,Goss M,Machalski M

更新日期：2000-11-01 00:00:00

abstract：:The family of chromosomal high mobility group I/Y consists of proteins involved in gene regulation and chromatin organization. A common feature of these proteins is the presence of multiple copies of DNA binding domains (DBD), the so-called AT-hooks. Bi- or tridendate interaction of two or three DBDs with appropriatel...

journal_title：International journal of molecular medicine

authors： Wisniewski JR,Schwanbeck R

更新日期：2000-10-01 00:00:00